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| Registros recuperados: 92 | |
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| Boudry, Pierre; Chatain, Beatrice; Naciri-graven, Yamama; Lemaire, Christophe; Gerard, Andre. |
| In France, marine fish and shellfish farming is based mainly on the breeding of wild species, whose natural populations are traditionally exploited. Research programs are conducted by IFREMER, in co-operation with INRA and Universities. French research programs for the genetical improvement of marine fish and shellfish concern primarily seabass (Dicentrarchus labrax) and oysters (Ostrea edulis and Crassostrea gigas). The following programs are developed: The knowledge of wild and farmed populations. The most recent prodjects involve the highly variable microsatellite markers. Seven markers are presently available for seabass and the work is now initiated on turbot (Scophthalmus maximus). In molluscs, microsatellite markers have been developed for the... |
| Tipo: Text |
Palavras-chave: Selection; Triploid; Shellfish; Fish; Genetic. |
| Ano: 1996 |
URL: http://archimer.ifremer.fr/doc/1996/acte-3206.pdf |
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| Renault, Tristan; Stokes, Nancy; Chollet, Bruno; Cochennec, Nathalie; Berthe, Franck; Gerard, Andre; Burreson, Eugene. |
| Two cases of haplosporidian infection occurred during 1993 in Pacific oysters Crassostrea gigas from the French Atlantic coast. The localization and ultrastructure of the plasmodia are described. In situ hybridization of infected tissue sections was conducted with DNA probes for oyster-infecting haplosporidians. The Haplosporidium nelsoni-specific DNA probe MSX1347 hybridized with the C. gigas parasite, and the H, costale-specific probe SSO1318 did not hybridize. Total genomic DNA was extracted from the infected tissue sections for polymerase chain reaction (PCR) amplification of the haplosporidian. PCR amplifications with H. nelsoni-specific primers and with 'universal' actin primers did not yield the expected products of 573 and 700 bp, respectively. A... |
| Tipo: Text |
Palavras-chave: Haplosporidium nelsoni; Haplosporidiosis; Crassostrea gigas; Pacific oyster. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/publication-821.pdf |
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| Fleury, Pierre-gildas; Carval, Jean-pierre; Muzellec, Marie-louise; Gerard, Andre; Barret, Jean; Cochard, Jean-claude; Dao, Jean-claude. |
| L'aquaculture et la pêche ne font pas toujours bon ménage : conflit pour les zones de production (élevages en mer), concurrence sur le marché (poisson d'élevage / poisson "sauvage"), et surtout conflit sur la gestion des stocks naturels de juvéniles, parfois exploités pour l'approvisionnement des élevages au détriment d'une production naturelle d'adultes pour le stock exploité par pêche (palourde du golfe du Morbihan, crevettes tropicales, etc). En d'autres cas les techniques d'élevage peuvent se révéler au contraire un outil précieux pour la gestion des stocks naturels, particulièrement pour des animaux plus ou moins sédentaires comme les Mollusques ou les Crustacés. Ici l'élevage des juvéniles va permettre de conforter le stock naturel des animaux... |
| Tipo: Text |
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| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/rapport-7308.pdf |
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| Barre, Marc; Naciri, Yamama; Boudry, Pierre; Goyard, Emmanuel; Launey, Sophie; Cochennec, Nathalie; Heurtebise, Serge; Ledu, Christophe; Phelipot, Pascal; Gerard, Andre. |
| The Aquaculture and Pathological Genetics (GAP) laboratory at La Tremblade has been monitoring three populations of European flat oyster since 1985 or 1989 under selection for higher survival rates in the presence of the parasite Bonamia ostrea. One selection cycle (1985 population) or two (1989 populations) were carried out by mass selection by placing the oysters for several years in a highly infested zone, and then harvesting spawns from survivors. |
| Tipo: Text |
Palavras-chave: Huîtres plates; Génétique; Selection génétique; Ostrea edulis. |
| Ano: 1997 |
URL: http://archimer.ifremer.fr/doc/1997/acte-3244.pdf |
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| Gerard, Andre; Naciri, Yamama; Peignon, Jean-marie; Ledu, Christophe; Phelipot, Pascal; Noiret, Christophe; Peudenier, Isabelle; Grizel, Henri. |
| For determination of ploidy levels in bivalves, three techniques are now routinely used: karyological determination. microfluorimetry and flow cytometry. This paper proposes an alternative technique which is based on determination of the optical density (OD) of stained nuclei using image analysis. A karyological and image analysis comparative experiment conducted on juveniles of Crassostrea gigas (Thunberg) and Ruditapes philippinarum (Adam & Reeve) is described and highlights the advantages of the image analysis technique. Initially developed on C. gigas and R. philippinarum, the image analysis method was easily extended to other bivalve species and to different developmental stages, as demonstrated by two experiments conducted on Ostrea edulis (L.).... |
| Tipo: Text |
Palavras-chave: Estimation bivalve triploidy; Image analysis; Triploid; Genetic; Crassostrea gigas; Oysters. |
| Ano: 1994 |
URL: http://archimer.ifremer.fr/doc/1994/publication-3200.pdf |
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| Boudry, Pierre; Collet, B; Mccombie, Helen; Ernande, Bruno; Morand, B; Heurtebise, Serge; Gerard, Andre. |
| In order to study individual growth variability and its relationship with survival in juvenile Crassostrea gigas, parental oysters were sampled at four sites located along the French Atlantic coast and bred under controlled hatchery conditions. Four groups of larvae were obtained by crossing five males and five females from each of the four sites, and a fifth group by crossing these 20 males and 20 females together in a pool. Larvae were reared under conditions allowing the maintenance of a maximum variability of size and gave five experimental groups. Oysters were individually monitored for growth and survival from 3 to 10 months after fertilization. The individual growth performances were relatively stable over time and no noticeable compensation for... |
| Tipo: Text |
Palavras-chave: Survival; Oysters; Growth; Crassostrea gigas; Aquaculture. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/publication-467.pdf |
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| Gerard, Andre; Naciri, Yamama; Noiret, Christophe; Ledu, Christophe; Peignon, Jean-marie. |
| For the first time, effective treatments using cytochalasin B were developed to induce triploidy in the European clam, Ruditapes decussatus (L.). The percentage of triploid embrios was assessed by karyological or image analysis. Two treatments (O.5 or 1 mg of cytochasin B (CB per ml of dimethylsulfoxide in 1 l of sea water) were applied at different times after fertilization (10 to 25 mn), for two different periods (15 and 20 min). Best results were obtained for a CB concentration of 1 mg/l. When treatment was applied 15 min after fertilization for a 20 min period, 94% and 95% of triploid embryos were obtained in two repeated experiments. At metamorphosis, the treated larvae appeared to be no smaller than the control larvae in all experiments. Thowever, in... |
| Tipo: Text |
Palavras-chave: Genetic; Ruditapes decussatus; Clam; Induced triploidy. |
| Ano: 1994 |
URL: http://archimer.ifremer.fr/doc/1994/publication-3208.pdf |
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| Naciri-graven, Y; Launey, Sophie; Lebayon, N; Gerard, Andre; Baud, Jean-pierre. |
| Genetic variability for growth was analysed in three populations of Ostrea edulis, selected for resistance to the protozoan parasite Bonamia ostreae. This study was undertaken first to determine the potential for selection for growth in populations that have never been selected for this character, and second to estimate heterosis versus inbreeding depression. Growth was monitored in culture for 10 months. The selected populations (namely S85-G3, S89I-G2 and S89W-G2), their crossbred population and a control population were composed of full-sib families whose parents were already genotyped using five microsatellite markers. This genotyping allowed the estimation of genetic relatedness among pairs of parents. The parents' relatedness was then correlated with... |
| Tipo: Text |
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| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/publication-2719.pdf |
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| Amiard, Jean-claude; Perrein Ettajani, H; Gerard, Andre; Baud, Jean-pierre; Amiard Triquet, C. |
| The present study was designed to compare the response to contaminants in diploid with triploid specimens of the oyster Crassostrea gigas. The reproduction investment in bivalve molluscs has priority on somatic growth. Thus, genetic sterilization by triploidy induction enables the energy flux to be directed toward somatic growth and glucide storage. Bioaccumulation was examined for Ag (10 mug/L), Cd (10 mug/L), and Cu (30 mug/L) to determine if the response to metals follows similar patterns in diploid (2n) and triploid (3n) groups. The effect of ploidy was also evaluated as a function of dry weight of soft tissue and condition index. Moreover, the reciprocal influence of these metals on their incorporation was studied. The results showed that the major... |
| Tipo: Text |
Palavras-chave: Oyster culture; Metal bioaccaumulation; Polyploidy. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-791.pdf |
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| Huvet, Arnaud; Gerard, Andre; Ledu, Christophe; Phelipot, Pascal; Heurtebise, Serge; Boudry, Pierre. |
| The distinction of the two cupped oysters Crassostrea gigas (Thunberg, 1793) and Crassostrea angulata (Lamark, 1819) into two species was chiefly due to their differing geographical distributions, C. gigas being present in Asia and C. angulata in Europe. Today it is commonly accepted that C. angulata and C. gigas are a single species according to morphological, genetic and F1 hybridization data. However, the demonstration of the fertility of their hybrids and the absence of any reproductive isolation remained to be investigated. Consequently, we studied the fertility of hybrids and sperm competition by performing three different experiments and producing G1 and G2 hybrid progenies between wild populations of C. angulata and C. gigas. Progenies showed very... |
| Tipo: Text |
Palavras-chave: Crassostrea angulata; Crassostrea gigas; Developmental yields of larvae; Fertility; Controlled crosses; Hybridization. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/publication-1395.pdf |
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| Bedier, Edouard; Baud, Jean-pierre; Cochennec, Nathalie; Cornette, Florence; Gerard, Andre; Goyard, Emmanuel; Joly, Jean-pierre; Kopp, Joel; Langlade, Aime; Le Coguic, Yvette; Mazurie, Joseph; Nourry, Max. |
| The European flat oyster Ostrea edulis has been considered as the traditional French oyster for a very long time and has now nearly disappeared from our farms, as a consequence of the two successive epizootic of the Marteilia refringens parasite and Bonamia ostreae. The actual quasi monoculture of Crassostrea gigas has made necessary the search for a diversification in oyster culture, including the search for a new start of the indigenous flat oyster culture. The Ifremer has been conducting parallel works since 1985 in order to better understand the biology of the parasite as well as to try and select different lines of flat oyster resistant to Bonamia ostreae. |
| Tipo: Text |
Palavras-chave: Bonamia ostrea; Huître plate; Sélection Génétique; Ostrea edulis. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/acte-3274.pdf |
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| Gerard, Andre; Thouard, Emmanuel; Veron, Gerard; Denis, Jacques; Thebaud, Olivier. |
| The "Artificial reefs" and "Repopulating" issues have been tackled by the Ifremer for a number of years without reaching a clear cut point of view, mainly for lack of socio-economic elements necessary to a rigorous analysis of these devices and practices. During a mission in Japan in January 2006, some Japanese experts, have presented to the head of the Ifremer a few examples of realisations related to these fields and conducted in their country. The head of the Ifremer realised the scope of such devices in Japan and expressed the wish to see an in-depth study carried out over there. In this purpose, the International Relationship Department organised and co-financed the three-month stay in Japan of a student from the University of Montpellier III, Sylvain... |
| Tipo: Text |
Palavras-chave: France; Etranger; Recommandations; Réglementation; Cadre juridique; Protection; Aménagement; Intérêt; Ecosystème; Récifs artificiels. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/rapport-6533.pdf |
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| Gerard, Andre; Naciri, Yamama; Peignon, Jean-marie; Ledu, Christophe; Phelipot, Pascal; Bodoy, Alain; Heurtebise, Serge; Garnier, Jacqueline; Baud, Jean-pierre; Nourry, Max; Haure, Joel; Martin, Anne-genevieve; Claude, Serge; Barret, Jean; Devauchelle, Nicole; Joly, Jean-pierre; Goulletquer, Philippe; Coatanea, Denis; Oheix, Jocelyne; Zanette, Yvan; Blachier, Philippe. |
| This fourth part of the polyploidisation program reports the experiments that were conducted in 1992 and that were devoted to : - the acquiring of a new method using an epifluroescent microscope - the development o a new technique for inducing polyploidy - the triploidy induction for the Europena clam Ruditapes decussatus - the settlement of performance controls conducted on diploid and triploid populations of Ostrea edulis - results of performance controls conducted on diploid and triploid populations of Crassostrea gigas and Ruditapes philippinarum |
| Tipo: Text |
Palavras-chave: Ruditapes decussatus; Ruditapes philippinarum; Ostrea edulis; Crassostra gigas; Cytongenetics; Polyploidy; Ruditapes decussatus; Ruditapes philippinarum; Ostrea edulis; Crassostrea gigas; Cytogénétique; Polyploïdie. |
| Ano: 1993 |
URL: http://archimer.ifremer.fr/doc/1993/rapport-3859.PDF |
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| Gerard, Andre; Naciri, Yamama; Peignon, Jean-marie; Ledu, Christophe; Phelipot, Pascal; Baud, Jean-pierre; Nourry, Max; Haure, Joel; Heurtebise, Serge; Garnier, Jacqueline; Martin, Anne-genevieve; Claude, Serge; Langlade, Aime; Joly, Jean-pierre; Goulletquer, Philippe; Ruelle, Francois; Coatanea, Denis; Oheix, Jocelyne; Vercelli, Catherine; Chabirand, Jean-michel; Maurer, Daniele; Borel, Michel; Cardinal, Mireille; Cornet, Josiane; Le Pennec, Marcel; Le Mercier, A.. |
| This fifth part of the polyploidisation program reports the experiments that were conducted in 1993 and that were devoted to : - monitoring growth performance of Ostrea edulis diploids and triploids in La Trinité sur Mer and Palavas - monitoring growth performance of Crassostrea gigas diploids and triploids in Port en Bessin, Marennes Oleron (2 locations), Arcachon and Palavas - comparative study of gametogenesis for C. gigas diploids and triploids - comparative study of C. gigas diploids and triploids using sensorial analysis At the end of fifth year, it was clear that triploids are advantageous although such a statement must be moderated according to locations. The gametogenesis stydy showed that triploidy is associated with partial to complete... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Ostrea edulis; Cytogenetics; Polyploidy; Crassostra gigas; Ostrea edulis; Cytogénétique; Polyploïdie. |
| Ano: 1994 |
URL: http://archimer.ifremer.fr/doc/1994/rapport-3860.PDF |
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| Gerard, Andre; Naciri, Yamama; Peignon, Jean-marie; Ledu, Christophe. |
| A novel method using 6-dimcthylaminopurinc (6-0MAP) has recently been demonstrated to be a good triploid inductor for bivalve molluscs. The effects of 6-DMAP concentration, timing of treatment after fertilization and treatment duration on survival and triploid induction of Crassostrea gigas (Thunberg) embryos were examined. An influence of sperm concentration was also detected and discussed. Survival to D-stage was inversely related to 6-DMAP concentration and the percentage of triploids was shown to be 6-DMAP dose dependent. Two optimal moments for treatment application were determined corresponding to extrusions of the first and second polar bodies. Optimal treatment of 450 µmol/l 6-DMAP beginning 15 min after fertilization over a 10 min period yielded... |
| Tipo: Text |
Palavras-chave: Genetic; Triploid induction; Crassostrea gigas; 6 DMAP; Triploid; Oysters. |
| Ano: 1994 |
URL: http://archimer.ifremer.fr/doc/1994/publication-3198.pdf |
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| Registros recuperados: 92 | |
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